PREPARATION OF DIPHOSPHOPYRIDINE NUCLEOTIDE
نویسندگان
چکیده
منابع مشابه
The preparation of diphosphopyridine nucleotide with ion exchange resin.
The older methods for the preparation of diphosphopyridine nucleotide (DPN) were reviewed in 1942 by Schlenk (1). In 1947 Sumner et al. (2) outlined the need for a greatly simplified method for the preparation of this coenzyme. These authors described a procedure which did away with heavy metal fractionation. Recently, Clark et al. (3) made several modifications in the above method. The LePage ...
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Enzymatic splitting of diphosphopyridine nucleotide (DPN)’ by microorganisms, plants, and animal tissues has been known for many years, but the precise nature of this reaction is not well understood (1). Ohlmeyer (2) first observed that adenylic acid (5-phosphoadenosine) appeared when DPN was incubated with yeast maceration juice. Later Heiwinkel (3) identified adenylic acid as a product of the...
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The need for additional study on the specific relation between metallic ions and metabolic function, especially in the case of the diphosphopyridine nucleotide oxidase system, has been expressed by Mahler (1) and others. This is particularly indicated in view of the fact that a number of the known components of this system contain iron. While it is broadly concluded that iron compounds facilita...
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A continuous flow instrumental system of analysis, first described by Skeggs (l), was previously applied to the determination of phosphohexose isomerase activity and resulted in a method that was approximately eightfold as rapid as the manual procedure (2). The present paper is concerned with the application of this system of analysis to the determination of enzyme activities that utilize the d...
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In continuation of our investigation of liver diphosphopyridine nucleotide-linked aldehyde dehydrogenase (1)) we have undertaken a study of various classes of inhibitors of the enzyme. Interest in aldehyde oxidation has been increasing in recent years from several points of view. The classical problem of ethanol oxidation in viva still has many unresolved facets (Z), and the enzymes responsible...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1947
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(17)30921-3